𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Modification of the BrdU/H33258 technique for flow cytometry based on the pH-dependence of the fluorescence of H33258 stained DNA

✍ Scribed by W. Kroll

Book ID
104786785
Publisher
Springer
Year
1984
Tongue
English
Weight
798 KB
Volume
80
Category
Article
ISSN
1432-119X

No coin nor oath required. For personal study only.

✦ Synopsis

A simple analytical method is described for the evaluation of cell cycle progression data by modification of the BrdU/H33258 technique for flow cytometry. This procedure allows to obtain quenched and unquenched DNA-histograms of cells containing BrdU-substituted DNA by staining with one dye only, namely H33258. Quenched histograms are obtained at pH = 7 and give information about how far cells have passed the cell cycle since the beginning of the incubation. The unquenched ones obtained at pH less than or equal to 4 of the staining solution give information about the actual position of the cells in the cell cycle.

πŸ“œ SIMILAR VOLUMES

A rapid flow cytometry test based on his
A rapid flow cytometry test based on histone H2AX phosphorylation for the sensitive and specific diagnosis of ataxia telangiectasia
✍ Paola Porcedda; Valentina Turinetto; Alfredo Brusco; Simona Cavalieri; Erica Lan πŸ“‚ Article πŸ“… 2008 πŸ› John Wiley and Sons 🌐 English βš– 178 KB

## Abstract Ataxia telangiectasia (A‐T) is a progressive neurodegenerative disease with onset in early childhood, caused by mutations in the __ATM__ (ataxia‐telangiectasia mutated) gene. Diagnosis relies on laboratory tests showing high levels of serum alphafetoprotein, cell sensitivity to ionizing