Modification of native collagen reduces antigenicity but preserves cell compatibility
✍ Scribed by Janet Hardin-Young; Robert M. Carr; Gregory J. Downing; Kimberlie D. Condon; Paul L. Termin
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 901 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Porcine intestinal collagen (ICL), derived from processed small intestine, is used as a part of a remodelable bilaminate biosynthetic vascular prosthesis. The process for the production of ICL involves mechanical cleaning of noncrosslinked porcine intestine (NC-ICL), disinfection with peracetic acid (PA-ICL), and crosslinking with I-ethyl-3-(3dimethylaminopropyl) carbodiimide hydrochloride (PA/ EDC-ICL). Two model systems were investigated t o evaluate the effect of these agents on the humoral response to NC-ICL. First, the antibody titers of rabbits immunized with NC-ICL, PA-ICL, and PA/EDC-ICL were determined, and second, the humoral response of canines receiving collagenous vascular implants was examined. Collagenous and noncollagenous fractions were extracted from NC-ICL, PA-ICL, and PA/EDC-ICL and separated by SDS-PAGE. PA and EDC treatment decreased the number of extractable proteins as compared to NC-ICL. lmmunoblot techniques demonstrated anti-NC-ICL antibodies recognized mutliple immunoreactive proteins in NC-ICL, but not i n PA-ICL or PA/EDC-ICL; and rabbits immunized with NC-ICL produced higher antibody titers to ICL proteins than rabbits immunized with either PA-ICL or PNEDC-ICL. It was, therefore, apparent that NC-ICL was more antigenic than either PA-ICL or PA/EDC-ICL. The humoral immune response of canines to PA/EDC-ICL fabricated vascular grafts was determined. At 4 weeks, 8 weeks, and 12 weeks postimplant, serum antibodies to ICL proteins or type I collagen could not be detected. These data demonstrate a reduced humoral immune responseto PNEDC-ICL. 0