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Modification of carboxyl groups in the binding site of trypsin with the Meerwein reagent

✍ Scribed by Nakayama, Hitoshi; Tanizawa, Kazutaka; Kanaoka, Yuichi


Book ID
123125526
Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
272 KB
Volume
40
Category
Article
ISSN
0006-291X

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The usefulness of ricin as a research tool is handicapped by its extremely biohazardous nature. In this work, ricin toxicity has been reduced by chemical modification of carboxyl groups using 1-ethyl-3(3-dimethylaminopropyl) carbodiimide and [14C]glycine methyl ester. The reaction was carried out in