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Modification of available arginine residues in proteins by p-hydroxyphenylglyoxal

โœ Scribed by R.Bryan Yamasaki; Antonio Vega; Robert E. Feeney

Book ID
102985462
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
654 KB
Volume
109
Category
Article
ISSN
0003-2697

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Colorimetric determination of arginine r
Colorimetric determination of arginine residues in proteins by p-nitrophenylglyoxal
โœ R.Bryan Yamasaki; Dorothy A. Shimer; Robert E. Feeney ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 622 KB

A new method is presented for the calorimetric determination of arginine residues in proteins. Under mildly alkaline conditions, p-nitrophenylglyoxal reacted with arginine to produce a stable colored solution in the presence of 0.15 M sodium ascorbate. Complete color development was obtained after 3