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Modification of amino groups in Candida utilis uricase with naphthoquinone disulfonic acid in relation to the enzymic activity

✍ Scribed by Kazuo Yoshida; Hiroyuki Nishimura; Katsunobu Takahashi; Ayako Matsushima; Yuji Inada


Book ID
115912305
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
194 KB
Volume
134
Category
Article
ISSN
0014-5793

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## Abstract Covalent modification of purified __Trigonopsis variabilis__ D‐amino acid oxidase using maleimide‐activated poly(ethylene glycol) 5000 yielded a stable bioconjugate in which three surface‐exposed cysteine side chains were selectively derivatized. Compared with the native enzyme, the PEG