Modification of 40S ribosomal subunits from yeast with dimethylmaleic anhydride

✍ Scribed by Francisco Hernández; Agustín Vioque

• Book ID: 104768193
• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 718 KB
• Volume: 11
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00419733

✦ Synopsis

Modification of 40S ribosomal subunits from Saccharomyces cerevisiae with dimethylmaleic anhydride (DMMA*), a reagent for protein amino groups, is accompanied by loss of polypeptide-synthesizing activity and by dissociation of proteins from the particles. The protein-deficient ribosomal particles, originated from 40S subunits by treatment with dimethylmaleic anhydride at a molar ratio of reagent to particle of 250, can partially reconstitute active subunits upon addition of the corresponding released proteins, and regeneration of the modified amino groups.