Modern microscopical imaging methods for the localisation of VIP mRNA, translated protein and binding (receptors) sites

Filter assay method for the estrogen rec

Filter assay method for the estrogen receptor protein: Detection of both filled and unfilled estrogen binding sites

✍ Lawrence Cousens; Barnet Eskin 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 829 KB

New filter assay methods are presented for quantitating both cytoplasmic and nuclear forms of the estrogen receptor protein. These methods exploit the strong adsorption of this protein to glass-fiber filters, which appears to occur without loss of steroid binding affinity. A "direct assay protocol"