Modern Biocatalysis || Importance of Enzyme Formulation for the Activity and Enantioselectivity of Lipases in Organic Solvents

✍ Scribed by Fessner, Wolf-Dieter; Anthonsen, Thorleif

• Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
• Year: 2008
• Tongue: German
• Weight: 156 KB
• Edition: 1
• Category: Article
• ISBN: 3527320717
• DOI: 10.1002/9783527623839.ch5

✦ Synopsis

5 5.1 a Relative to crude lipase BC taken as 1.

b The transesterifi cation between 1 -octanol (0.19 M) and vinyl butyrate (0.79 M) to give 1 -octyl butyrate and acetaldehyde was used as a model reaction. An amount of enzyme form containing 10 µ g of protein was used in a reaction volume of 1 ml. The activity of crude lipase BC was 0.5 µ mol/min × 10 µ g of protein. c PEG, methoxy poly(ethylene glycol) (molecular mass 5000 Da). d Lipase BC as commercialized by AMANO company (commercial name lipase PS). e CLEC, cross -linked enzyme crystals. Sample obtained as a kind gift of Altus. f Lipase BC entrapped in sol gel -AK. Sample purchased from Fluka.