The kinetic behavior of soluble and insoluble forms of dextransucrase from Leuconostoc mesenteroides NRRL B-1299 was investigated with sucrose as sub- strate and maltose as acceptor. To study the parameters involved, a kinetic model was applied that was previously developed for L. mesenteroides NRRL
Modelling of oligosaccharide synthesis by dextransucrase
✍ Scribed by B. Demuth; H. J. Jördening; K. Buchholz
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 434 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Dextransucrase catalyses the formation of dextran, but also of numerous oligosaccharides from sucrose and different acceptors, if appropriate conditions are chosen. Much experimental work has been carried out and a scheme of reactions and a mathematical model have been developed to describe the complex kinetic behaviour of the enzyme. A computer program was used to calculate the parameters of the model from a broad range of experimental data, investigating a large number of kinetic tests with the acceptors maltose and fructose. The results lead to design considerations for a continuous reactor system with immobilized dextransucrase to produce leucrose, a disaccharide of industrial interest.
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## Abstract Co‐Immobilization of dextransucrase (DS) and dextranase (DN) into calcium alginate includes the co‐entrapment of soluble DS and adsorbed DN. DS converts sucrose into dextran, which is the substrate for DN, so that isomalto‐oligosaccharides (IMOs) are follow‐up products of dextran hydrol
ty, efficiency and operational stability. The immobilization mechanism was investigated and the reaction conditions for leucrose formation were optimized. A surprising aspect was the finding, that yields and selectivity were much improved by reactions in ice. Referring to technical application of le