The transport of D-glucose in liposome dispersions prepared from lecithin-dicetyl phosphate-cholesterol was studied. The transport experiments were carried out using previously developed techniques in which the release of the solute is studied from dispersions contained in dialysis bags. The experimental results of the direct-release and uptake-release experiments were analyzed by the monosize, multiconcentric layer Model 2, which was developed and used recently for the 3-0-methyl-D-glucose transport studies. Uniformly good fits of the experimental data with the model were observed, and the permeability coefficients and the aqueous-lipid partition coefficients were calculated for liposome dispersions containing different percentages of cholesterol. A direct relationship between the percent cholesterol in liposome dispersions and the effective bulk permeability coefficient was ohtained. The incorporation of cholesterol into liposome dispersions does not seem to alter the effective aqueous-lipid partition coefficient.
Keyphrases 0 Lecithin spherules-transport of D-glucose in lecithin-dicetyl phosphate-cholesterol spherules, permeability and partition coefficients 0 D-Glucose-transport in lecithin-dicetyl phosphate-cholesterol spherules, permeability and partition coefficients 0 Cholesterol-transport of D-glucose in lecithin-dicetyl phosphate-cholesterol spherules, permeability and partition coefficients 0 Permeability-lecithin-dicetyl phosphate-cholesterol liposome dispersions, model transport studies Partition coefficients-lecithin-dicetyl phosphate-cholesterol liposome dispersions, model transport studies
In previous reports (1-3), quantitative methods were developed and used in the determination of effective bulk permeability coefficients for D-glucose, 3-O-methyl-D-glucose, and taurocholic acid-[cholic-