✦ LIBER ✦

Model of murine interpositional vein grafting

✍ Scribed by Brian C. Cooley

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 166 KB
Volume: 25
Category: Article
ISSN: 0738-1085
DOI: 10.1002/micr.20106

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A new murine model is presented for interpositional vein grafting into an artery. In ICR (outbred) mice, the femoral vein or a branch of the jugular vein was harvested and placed into a femoral artery defect, using standard microvascular techniques to achieve end‐to‐end anastomoses with 11‐0 nylon suture. The technique requires a high level of microsurgical experience and competence. Success rates (vessel patency) were higher with moderate systemic heparinization (200 units/kg body weight, single i.v. bolus). Vein grafts were successfully transplanted between syngenic C57Bl/6J mice, with patency demonstrated from 1 day to 3 months postoperatively. The model is applicable to studies of vein graft stenosis using murine transgenic/knockout models. © 2005 Wiley‐Liss, Inc. Microsurgery 25:00–00 2005.

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