✦ LIBER ✦

Model nucleoproteins: Binding of actinomycin D to complexes of DNA with lysine-containing polypeptides

✍ Scribed by H. Votavoá; K. Bláha; J. Šponar

Publisher: Wiley (John Wiley & Sons)
Year: 1978
Tongue: English
Weight: 687 KB
Volume: 17
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1978.360170710

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Complexes of DNA with histone H1 and random and sequential polypeptides containing 30–100% of lysine were studied using actinomycin D as a probe. The binding of actinomycin D was measured by spectrophotometric titration in 0.15__M__ NaCl and in 0.01__M__ Tris buffer. The excluded‐site model was used for the evaluation of binding data. Polypeptides reduce the number of binding sites on DNA available for actinomycin D binding. The extent of this change depends mainly on the content and distribution of basic lysine residues. Of the hydrophobic residues constituting the peptides, only leucine strongly depresses the actinomycin D binding. The helix‐forming and helix‐breaking amino acid residues are without effect.

📜 SIMILAR VOLUMES

7-Amino-actinomycin D complexes with deo

7-Amino-actinomycin D complexes with deoxynucleotides as models for the binding of the drug to DNA

✍ Yu-Chih Chen Chiao; K. Gurudath Rao; John W. Hook III; Thomas R. Krugh; Sisir K. 📂 Article 📅 1979 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 798 KB

## Abstract Fluorescence, CD, absorption, and ^1^H‐nmr studies are reported for complexes of 7‐amino‐actinomycin D with deoxydinucleotides, deoxytetranucleotides, and poly(__d__G‐__d__C)· poly(__d__G‐__d__C). The optical spectra for the 7‐amino‐actinomycin D complex with p__d__G‐__d__C, p__d__G‐__d