Model for the production of L-tryptophan from L-serine and indole by immobilized cells in a three-phase liquid-impelled loop reactor
✍ Scribed by D. M. R. Mateus; S. S. Alves; M. M. R. Fonseca
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 698 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1615-7605
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✦ Synopsis
Production of L-tryptophan from L-serine and a~ indole catalyzed by Escherichia coli, immobilized in kcarrageenan gel beads, is technically feasible in the liquid-Ay impelled loop reactor (LLR), using an organic solvent, e.g.
Cb n-dodecane.
With L-serine in large excess intrinsic reaction kinetics is Ce approximately first order with respect to indole, with a reaction Ca constant of 8.5 x 10 -~ m3 kgdw-~ s -1.
The overall process kinetics is jointly controlled by intrinsic C~s kinetics and by intraparticle mass transfer resistance, which can be quantified using an effectiveness factor.
Cs Mass transfer of indole from the organic to the aqueous phase and from the aqueous to the gel phase are relatively fast d, e,f and thus have negligible influence in the overall process kinetics, under the operational conditions tested. However, dp they may become important if the process is intensified by /(2 increasing the cell concentration in the gel and/or the gel KI hold-up in the reactor.
KM
A simple model which includes indole mass balances over K x the aqueous and organic phases, mass transfer and reaction kinetics, with parameters experimentally determined in inde-Kxa x pendent experiments, was successful in simulating L-tryptophan production in the LLR. Ky
List of symbols a, b, c coefficients of the equilibrium curve for indole between organic and aqueous phases A, B, C, D, E, F auxiliary variables used in liquid-liquid mass transfer studies ax specific interfacial area referred to the volume of the aqueous phase (m -1) Ax interfacial area (m 2)