Mode of action of insect growth regulators in lepidopteran tissue culture

✍ Scribed by Oberlander, Herbert; Silhacek, Donald L.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 229 KB
• Volume: 54
• Category: Article
• ISSN: 1526-498X
• DOI: 10.1002/(sici)1096-9063(1998110)54:3<300::aid-ps830>3.0.co;2-8

✦ Synopsis

Insect growth regulators (IGRs) have been proposed as agents for the control of insect pests. These compounds disrupt the normal development of insects by mimicking juvenile hormone and the molting hormone, 20-hydroxyecdsyone, or by interfering with chitin synthesis. The e †ectiveness and selectivity of IGRs provide new tools for integrated pest management. The simultaneous advances in the chemistry of IGRs and the ability to study insect tissues in culture, have led to research on the mode of action of IGRs in vitro. Plodia interpunctella and Spodoptera frugiperda have been used to examine the e †ects of IGRs on wing imaginal discs in organ culture, as well as in hormonally responsive cell lines established from wing imaginal discs of these species. Our research has focused on the action of ecdysteroid mimics, chitin synthesis inhibitors and juvenile hormone mimics. The e †ects of the IGRs on chitin synthesis, uptake of amino-sugars, and cellular proliferation were studied in tissue culture. The results demonstrate the e †ectiveness of using organ cultures and hormonally responsive cell lines for investigating IGRs at the cellular and tissue level.