Mobility of single DNA chain under electric field during its transient contact with solid surfaces
✍ Scribed by Benxin Jing; Qingbo Yang; Yingxi Zhu; Jiang Zhao
- Book ID
- 105339287
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 216 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0887-6266
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✦ Synopsis
Abstract
The motion of DNA chain under electric field when it is in transient contact with the solid surfaces in aqueous solution was studied by single molecule fluorescence microscopy at the total internal reflection geometry (TIRFM). In situ observation discovered that single λ‐DNA chains driven by electric field made transient contact with the solid surface and made hitting–sliding–leaving‐like motion along the surface. By varying the surface chemistry, from the negative‐charged silanol group‐rich surface to positive‐charged amino group‐rich surface, as well as hydrophobic surfaces, the dependence of DNA mobility on the surface–DNA interaction was studied. The results show that a dependence of the mobility of DNA on the surface polarity with respect to DNA itself. The study on different surfaces rich of silanol, amide, amino, and methyl groups show a sequence of DNA mobility of silanol > amide > amino. The mobility of DNA on methyl terminated surface was found to be similar to that on amino surface. © 2009 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 47: 2541–2546, 2009