Mobile phase optimization of a urea-linked chiral stationary phase for the high-performance liquid chromatographic separation of optical isomers

✍ Scribed by Subhash C. Dhanesar; Daryl J. Gisch

• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 765 KB
• Volume: 461
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94307-3

✦ Synopsis

A silica-based urea-linked chiral stationary phase, (R)-N-(l-naphthylethyl-N'propylsilyl urea), was studied with binary, ternary, and single-component mobile phases, to determine the factors which most affect retention and selectivity. Binary mobile phases were prepared with hexane as the non-selective solvent, and 2-propanol, dichloromethane, or chloroform as the selective solvent. ( f )-l-Phenylethyl-3,5dinitrobenzamide was used as the enantiomer test mixture. Increasing the mobile phase strength reduced the retention of test solutes, while selectivity remained largely unchanged. Also, modifiers improved resolution and did not affect selectivity. Single-component mobile phases reduced the retention in the order of increasing solvent strength, selectivity being solvent-dependent.