MLL/SEPTIN6 chimeric transcript from inv ins(X;11)(q24;q23q13) in acute monocytic leukemia: Report of a case and review of the literature

✍ Scribed by Hee-Jin Kim; Chang-Seok Ki; Quehn Park; Hong-Hoe Koo; Keon-Hee Yoo; Eun-Jeong Kim; Sun-Hee Kim

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 339 KB
• Volume: 38
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.10235

✦ Synopsis

Abstract

Rearrangements of the MLL gene on chromosome 11, band q23, are one of the most common genetic changes in acute leukemia. Reciprocal translocation is the most common form of MLL rearrangement, and the partner genes in MLL translocation are notably diverse. Involvement of the SEPTIN6 gene on Xq24 in MLL rearrangements occurs very rarely, with only six cases having been documented in the literature. Of note, the MLL/SEPTIN6 rearrangements in these cases were cryptic or complex, and it was shown that the 5′‐MLL/SEPTIN6‐3′ transcript resides on the derivative X chromosome rather than on the derivative chromosome 11 as in the majority of cases of MLL translocations. These observations suggested that MLL and SEPTIN6 reside on their respective chromosome loci in reverse orientation, that is, centromere‐to‐telomere and telomere‐to‐centromere, respectively. We here report a case of acute monocytic leukemia with inv ins(X;11)(q24;q23q13) in a 29‐month‐old child. Fluorescence in situ hybridization study revealed the break‐apart 5′‐MLL segment to be translocated to the derivative X chromosome, and reverse transcriptase–polymerase chain reaction followed by sequencing analysis confirmed the 5′‐MLL/SEPTIN6‐3′ chimeric transcript. This case is the first to provide direct cytogenetic evidence for the salient nature of the MLL/SEPTIN6 rearrangement. We reviewed clinical and cytogenetic features of all cases of 11q23 and Xq22–24 rearrangements reported up to now, including six cases where the involvement of the SEPTIN6 gene was confirmed by molecular techniques. © 2003 Wiley‐Liss, Inc.