Mixed-mode hydrophobic ion exchange for the separation of oligonucleotides and DNA fragments using HPLC
✍ Scribed by Thomas R. Floyd; Susan E. Cicero; Steven D. Fazio; Thomas V. Raglione; Shih-Hsien Hsu; Stephen A. Winkle; Richard A. Hartwick
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 636 KB
- Volume
- 154
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Hydrophobic anion exchangers were formed by cobonding both ionic and hydrophobic ligands to silica gel. These phases were used to separate single-stranded oligonucleotides and double-stranded DNA restriction fragments. By varying the ratio of n-octyldimethylsilane and either 3-chloropropyldimethylsilane or 4-chlorobutyldimethylsilane added during silanization a series of mixed-ligand or mixed-mode stationary phases was created. Concentration and ratio of bonded ligands were determined using a new gas chromatography fluorination method. Total ligand coverage was found to approach 2.1 ligands nm-2 for n-octyldimethylsilane. Bonding reproducibility for mixed-mode phases was good. Nucleic acid separations were achieved under gentle mobile phase conditions by using the stationary phase as an easily modifiable variable.