henic acid and to form a mixed LB film in which GOx The formation of behenic acid monolayers at the air/water interis active. However, some problems of reproducibility were face has been studied in the presence of glucose oxidase. When encountered, leading to differences in the slope of the calithe enzyme is present in the subphase, spreading of behenic acid bration curves from one biosensor to another. After improvat the air/water interface leads to the formation of a mixed film ing the electrochemical part of the biosensor, we found that of glucose oxidase and behenic acid. The pressure-area isotherms this poor reproducibility of the calibration curves was also performed with various concentrations of the enzyme in the subdue to events occurring during the preparation and the transphase showed the incorporation of the enzyme in the monolayer.
fer of the mixed films. Therefore, we decided to study in a
The dynamics of the mixed films was studied by following the more detailed way the formation of the mixed GOx/behenic evolution of the film area at a constant pressure and the evolution of the pressure at a constant area. Glucose oxidase dramatically acid film at the air/water interface in order to better understabilizes the behenic acid film. A limit pressure of incorporation stand the process. The transfer is also an important step in of glucose oxidase in the behenic acid film at the air/water interthe formation of the LB film but the formation of the mixed face was observed at 28 m N/m.