Mitotic instability of integrated plasmids in Penicillium chrysogenum transformants
β Scribed by D.V. Renno; G. Saunders; P. Smith; A.T. Bull; G. Holt
- Book ID
- 119131723
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 457 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0168-1656
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Large (10.5-13.5 kbp) circular minichromosomes containing the centromere of chromosome 11 (CEN11) and the MET14 gene of Saccharomyces cerevisiae in the YRp7 vector are considerably more stable during mitosis than smaller ones containing only the 1.6 kbp CEN11 SalI-fragment. Yeast transformants obtai