Mitomycin-C induced improvement in superficial bladder tumors: A morphologic and immunohistochemical evaluation

✍ Scribed by Strobel, S.L. ;Tuttle, S.E. ;Wise, H.A. ;Sharma, H.M.

• Book ID: 104689173
• Publisher: Springer
• Year: 1985
• Tongue: English
• Weight: 372 KB
• Volume: 13
• Category: Article
• ISSN: 0300-5623
• DOI: 10.1007/bf00261565

✦ Synopsis

Twenty bladder biopsies from ten patients with superficial transitional cell carcinoma were obtained prior to and following therapy with intravesical mitomycin-C. Each biopsy was evaluated histologically and immunohistochemically for A, B, H blood group antigen (BGA) expression. A, B and H blood group antigens were identified using monoclonal anti-A, B and H antibody and were localized with the avidin-biotin-peroxidase complex (ABC) technique. Particular attention was directed toward flat urothelial lesions and the urothelium adjacent to papillary tumors. Routine histologic evaluation showed improvement in post-therapy biopsies compared to corresponding pre-therapy biopsies in 7/10 cases but was equivocal in 3/10 cases. Immunohistochemical evaluation, however, showed improvement in all 10 cases, as judged by increased urothelial BGA expression. This increase in urothelial BGA expression after intravesical mitomycin-C therapy suggests a therapy induced improvement in dysplastic urothelium which was not uniformly evident on routine histologic examination.