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Mitogenic activity of procathepsin D purified from conditioned medium of breast-cancer cells by affinity chromatography on pepstatinyl agarose

✍ Scribed by A. J. Stewart; N. H. Piggott; F. E. B. May; B. R. Westley

Book ID: 102866863
Publisher: John Wiley and Sons
Year: 1994
Tongue: French
Weight: 599 KB
Volume: 57
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910570518

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✦ Synopsis

A simple procedure for the affinity purification of procathepsin D from tissue culture medium conditioned by breast-cancer cells is described. This procedure yielded 2 pg of procathepsin D/ I00 ml medium. The procathepsin D was approximately 95% pure as judged by silver staining of polyacrylamide gels, the major contaminant being mature cathepsin D. The ability of procathepsin D to stimulate the proliferation of oestrogenresponsive MCF-7 breast cancer cells was determined. The purified procathepsin D had no mitogenic effect alone or in combination with oestradiol or other growth factors. These data suggest that procathepsin D does not act as an oestrogenregulated autocrine growth factor for malignant breast epithelial calls.

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