✦ LIBER ✦

Mitogenic activity of insulin in the culture of a trypanosome: duration and dose response

✍ Scribed by David Mackintosh; Kim Coleman; Angela J Davies

Book ID: 104375222
Publisher: Elsevier Science
Year: 2003
Tongue: English
Weight: 84 KB
Volume: 27
Category: Article
ISSN: 1065-6995
DOI: 10.1016/s1065-6995(02)00251-2

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✦ Synopsis

Abstract

The effects of insulin on the in vitro growth characteristics of Trypanosoma granulosum were investigated. The insulin growth stimulus had a rapid onset with little or no lag phase but was relatively short lived, growth peaking typically on day 3 or 4 of culture. This contrasted with medium containing 10% foetal calf serum, normally used for in vitro culture of this trypanosome, which after a 3 day lag stimulated sustained log‐phase growth. Insulin demonstrated a biphasic dose response with maximum growth typically occurring at 3–10 μg/ml and a much reduced effect at higher concentrations. These findings suggest a possible role for host insulin and/or trypanosomal insulin‐like molecules in regulating parasite growth in vivo.

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