𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Mitogen activated protein (MAP) kinase activity is increased in adult mouse superior cervical ganglia during culturing

✍ Scribed by Bodil Svensson; Per A.R. Ekström

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
111 KB
Volume
52
Category
Article
ISSN
0360-4012

No coin nor oath required. For personal study only.

✦ Synopsis

We have used adult mouse superior cervical ganglia (SCG) to study the role of mitogen activated protein (MAP) kinase activity during axonal outgrowth in vitro. An initial peak in activity within the first hours of culture was followed by a substantially higher activity after 1 to 2 days, a time when axons were actively growing. The latter peak is probably a result of both higher levels of protein and increased activity. The addition of nerve growth factor stimulated both outgrowth and kinase activity, whereas treating the cultures with the kinase inhibitor PD98059 had an opposite effect. Taken together, the results suggest that activation of the MAP kinase pathway could be involved in the initiation as well as regulation of axonal outgrowth from adult SCG.

📜 SIMILAR VOLUMES

Mitogen-activated protein kinase inhibit
Mitogen-activated protein kinase inhibition reveals differences in signalling pathways activated by neurotrophin-3 and other growth-stimulating conditions of adult mouse dorsal root ganglia neurons
✍ Peter Wiklund; Per A.R. Ekström; Anders Edström 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 525 KB

## Abstract PD98059 blocks mitogen‐activated protein kinase (MAPK) by inhibiting its activator, MAP kinase kinase (MEK). We have previously found that PD98059 only transiently inhibits spontaneous axonal outgrowth from adult mouse dorsal root ganglia (DRG) explants, whereas it causes sustained inhi