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Mitochondrial release factor in yeast: interplay of functional domains

✍ Scribed by Jan Kutner; Joanna Towpik; Krzysztof Ginalski; Magdalena Boguta

Publisher
Springer-Verlag
Year
2008
Tongue
English
Weight
413 KB
Volume
53
Category
Article
ISSN
0172-8083

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In order to facilitate studies on protein localization to and sorting within yeast mitochondria, we have designed an experimental system that utilizes a new vector and a functional assay. The vector, which we call an LPS plasmid (for leader peptide substitution), employs a yeast COX% gene (the struc