Mitochondrial nitric oxide synthase drives redox signals for proliferation and quiescence in rat liver development
✍ Scribed by María C. Carreras; Daniela P. Converso; Alicia S. Lorenti; Mariana Barbich; Damián M. Levisman; Ariel Jaitovich; Valeria G. Antico Arciuch; Soledad Galli; Juan J. Poderoso
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 369 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0270-9139
No coin nor oath required. For personal study only.
✦ Synopsis
Mitochondrial nitric oxide synthase (mtNOS) is a fine regulator of oxygen uptake and reactive oxygen species that eventually modulates the activity of regulatory proteins and cell cycle progression. From this perspective, we examined liver mtNOS modulation and mitochondrial redox changes in developing rats from embryonic days 17-19 and postnatal day 2 (proliferating hepatocyte phenotype) through postnatal days 15-90 (quiescent phenotype). mtNOS expression and activity were almost undetectable in fetal liver, and progressively increased after birth by tenfold up to adult stage. NO-dependent mitochondrial hydrogen peroxide (H 2 O 2 ) production and Mn-superoxide dismutase followed the developmental modulation of mtNOS and contributed to parallel variations of cytosolic H 2 O 2 concentration ([H 2 O 2 ] ss ) and cell fluorescence. mtNOS-dependent [H 2 O 2 ] ss was a good predictor of extracellular signal-regulated kinase (ERK)/p38 activity ratio, cyclin D1, and tissue proliferation. At low 10 ؊11 -10 ؊12 M [H 2 O 2 ] ss , proliferating phenotypes had high cyclin D1 and phospho-ERK1/2 and low phospho-p38 mitogen-activated protein kinase, while at 10 ؊9 M [H 2 O 2 ] ss , quiescent phenotypes had the opposite pattern. Accordingly, leading postnatal day 2-isolated hepatocytes to embryo or adult redox conditions with H 2 O 2 or NO-H 2 O 2 scavengers, or with ERK inhibitor U0126, p38 inhibitor SB202190 or p38 activator anisomycin resulted in correlative changes of ERK/p38 activity ratio, cyclin D1 expression, and [ 3 H] thymidine incorporation in the cells. Accordingly, p38 inhibitor SB202190 or N-acetylcysteine prevented H 2 O 2 inhibitory effects on proliferation. In conclusion, the results suggest that a synchronized increase of mtNOS and derived H 2 O 2 operate on hepatocyte signaling pathways to support the liver developmental transition from proliferation to quiescence. (HEPATOLOGY 2004;40:157-166.