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Mitochondrial DNA genotypes in nuclear transfer-derived cloned sheep

✍ Scribed by Schon, Eric A.; Evans, Matthew J.; Gurer, Cagan; Loike, John D.; Wilmut, Ian; Schnieke, Angelika E.


Book ID
109494919
Publisher
Nature Publishing Group
Year
1999
Tongue
English
Weight
300 KB
Volume
23
Category
Article
ISSN
1061-4036

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## Background The mitochondrial DNA (mtDNA) of the cloned sheep "Dolly" and nine other ovine clones produced by somatic cell nuclear transfer (SCNT) was reported to consist only of recipient oocyte mtDNA without any detectable mtDNA contribution from the nucleus donor cell. In cattle, mouse and pig

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## Abstract Highly diverse results have been reported for mitochondrial DNA (mtDNA) hetero‐plasmy in nuclear‐transferred farm animals. In this study, we cloned genetically defined mice and investigated donor mtDNA inheritance following somatic cell cloning. Polymerase chain reaction (PCR) analysis