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Mitochondrial DNA-directed RNA polymerase from Saccharomyces cerevisae mitochondria

โœ Scribed by A.H. Scragg


Book ID
118852610
Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
250 KB
Volume
45
Category
Article
ISSN
0006-291X

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A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, a