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Mitochondrial DNA and suppressiveness of petite mutants inSaccharomyces cerevisiae

โœ Scribed by Georg Michaelis; Stephen Douglass; Ming-Jer Tsai; Richard S. Criddle

Publisher
Springer
Year
1971
Tongue
English
Weight
526 KB
Volume
5
Category
Article
ISSN
0006-2928

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โœฆ Synopsis

Ethidium bromide is known to be a powerful mutagen for the induction of eytoplasmically inherited petite mutations in yeast. The effect of ethidium bromide on the degree of suppressiveness of the induced mutants as a function of exposure time is described. The mitoehondrial DNA of 20 ethidium bromide-induced petite mutants has been studied to determine its absence or presence and its buoyant density. Ten mutants, in which we were not able to detect any mitochondrial DNA, were neutral petites. The 10 remaining mutants with mitoehondrial DNA simultaneously showed a measurable degree of suppressiveness. It was not possible to correlate the buoyant density of the mutant mitochondrial DNA with the degree of suppressiveness.

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