The critically acclaimed laboratory standard for forty years, <b>Methods in Enzymology</b> is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 2
Mitochondrial Biogenesis and Genetics Part B
✍ Scribed by Giuseppe M. Attardi, Anne Chomyn (Eds.)
- Publisher
- Academic Press
- Year
- 1996
- Tongue
- English
- Leaves
- 657
- Series
- Methods in Enzymology 264
- Edition
- 1
- Category
- Library
No coin nor oath required. For personal study only.
✦ Synopsis
The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
This volume covers methodology used in
Mitochondrial gene expression
Mitochondrial genetics and gene manipulation
Mitochondrial diseases and aging
✦ Table of Contents
Content:
Contributors to volume 264
Pages ix-xii
Preface
Pages xiii-xiv
Giuseppe M. Attardi, Anne Chomyn
Volumes in series
Pages xv-xxx
[1]In vivo footprinting of human mitochondrial DNA in cultured cell systems Original Research Article
Pages 3-11
Vicente Micol, Patricio FerГЎndez-Silva, Giusep Peattardi
[2]Genomic footprinting of mitochondrial DNA: I. In organello analysis of protein-mitochondrial DNA interactions in bovine mitochondria Original Research Article
Pages 12-22
Cort S. Madsen, Steven C. Ghivizzani, Chandramohan V. Ammini, Marcel R. Nelen, William W. Hauswirth
[3] Genomic footprinting of mitochondrial DNA: II. In Vivo analysis of protein-mitochondrial DNA interactions in Xenopus laevis eggs and embryos Original Research Article
Pages 23-36
Chandramohan V. Ammini, Steven C. Ghivizzani, Cort S. Madsen, William W. Hauswirth
[4] In Organello mitochondrial protein and RNA synthesis systems from Saccharomyces cereuisiae Original Research Article
Pages 36-42
Robert O. Poyton, Gary Bellus, Edward E. McKee, Kevin A. Sevarino, Bradley Goehring
[5] In Organello RNA synthesis system from HeLa cells Original Research Article
Pages 43-49
George L. Gaines III
[6] In Organello RNA synthesis system from mammalian liver and brain Original Research Article
Pages 50-57
JosГ©A. EnrГquez, Acisclo PГ©rez-Martos, Manuel J. LГіpez-PГ©rez, Julio Montoya
[7] Transcription in Vitro with Saccharomyces cerevisiae mitochondrial RNA polymerase Original Research Article
Pages 57-66
David A. Mangus, Judith A. Jaehning
[8] Reactions catalyzed by group II introns in Vitro Original Research Article
Pages 66-86
Philip S. Perlman, Mircea Podar
[9] Genetic and biochemical approaches for analysis of mitochondrial RNase P from Saccharomyces cerevisiae Original Research Article
Pages 86-99
Kathleen R. Groom, Yan Li Dang, Guo-Jian Gao, Yan Chun Lou, Nancy C. Martin, Carol A. Wise, Michael J. Morales
[10] RNA editing in trypanosomatid mitochondria Original Research Article
Pages 99-121
Larry Simpson, Georges C. Frech, Dmitri A. Maslov
[11] Purification of mitochondrial DNA from human cell cultures and placenta Original Research Article
Pages 122-128
Carlo Ausenda, Anne Chomyn
[12] Mitochondrial DNA transcription initiation and termination using mitochondrial lysates from cultured human cells Original Research Article
Pages 129-139
Patricio FernГЎndez-Silva, Vicente Micol, Giuseppe Attardi
[13] Mapping promoters in displacement-loop region of vertebrate mitochondrial DNA Original Research Article
Pages 139-148
Gerald S. Shadel, David A. Clayton
[14]Isolation and characterization of vertebrate mitochondrial transcription factor a homologs Original Research Article
Pages 149-158
Gerald S. Shadel, David A. Clayton
[15]Isolation and assay of mitochondrial transcription termination factor from human cells Original Research Article
Pages 158-173
Vicente Micol, Patricio Fernandez-Silva, Giuseppe Attardi
[16] Isolation of mitochondrial tRNAs from human cells Original Research Article
Pages 173-182
Michael P. King
[17] Analysis of aminoacylation of human mitochondrial tRNAs Original Research Article
Pages 183-196
JosГ©Antonio EnrГquez, Giuseppe Attardi
[18] In vivo labeling and analysis of human mitochondrial translation products Original Research Article
Pages 197-211
Anne Chomyn
[19] Mitochondrial protein synthesis in rat brain synaptosomes Original Research Article
Pages 211-217
Paola Loguercio Polosa, Giuseppe Attardi
[20] Immunoprecipitation of human mitochondrial translation products with peptide-specific antibodies Original Research Article
Pages 218-228
Herman A.C.M. Bentlage, Anne Chomyn
[21] Genetic strategies for identification of mitochondrial translation factors in Saccharomyces cerevisiae Original Research Article
Pages 228-237
Thomas D. Fox
[22] Bovine mitochondrial ribosomes Original Research Article
Pages 237-248
Thomas W. O'Brien, Nancy D. Denslow
[23] Bovine mitochondrial initiation and elongation factors Original Research Article
Pages 248-261
Caryl J. Schwartzbach, Mary Farwell, Hua-Xin Liao, Linda L. Spremulli
[24] Transformation of Saccharomyces cerevisiae mitochondria using the biolistic gun Original Research Article
Pages 265-278
Ronald A. Butow, R.Michael Henke, John V. Moran, Scott M. Belcher, Philip S. Perlman
[25] Genetics and transformation of mitochondria in the green alga Chlamydomonas Original Research Article
Pages 279-296
John E. Boynton, Nicholas W. Gillham
[26] Isolation of avian mitochondrial DNA-less cells Original Research Article
Pages 296-304
RГ©jean Morais
[27] Isolation of human cell lines lacking mitochondrial DNA Original Research Article
Pages 304-313
Michael P. King, Giuseppe Attardi
[28] Mitochondria-mediated transformation of human П±0 cells Original Research Article
Pages 313-334
Michael P. King, Giuseppe Attardi
[29] Platelet-mediated transformation of human mitochondrial DNA-less cells Original Research Article
Pages 334-339
Anne Chomyn
[30] Use of ethidium bromide to manipulate ratio of mutated and wild-type mitochondrial DNA in cultured cells Original Research Article
Pages 339-344
Michael P. King
[31]Construction of heteroplasmic mice containing two mitochondrial DNA genotypes by micromanipulation of single-cell embryos Original Research Article
Pages 345-357
Philip J. Laipis
[32] Mitochondria-mediated transformation of Drosophila Original Research Article
Pages 357-368
Etsuko T. Matsuura, Yuzo Niki
[33] Allotopic expression of mitochondrial ATP synthase genes in nucleus of Saccharomyces cerevisiae Original Research Article
Pages 369-389
Robyn E. Gray, Ruby H.P. Law, Rodney J. Devenish, Phillip Nagley
[34] Allotopic expression of yeast mitochondrial maturase to study mitochondrial import of hydrophobic proteins Original Research Article
Pages 389-403
Manuel G. Claros, Javier Perea, Claude Jacq
[35] Automated sequencing of mitochondrial DNA Original Research Article
Pages 407-421
Masashi Tanaka, Mika Hayakawa, Takayuki Ozawa
[36] Detection and quantification of mitochondrial DNA deletions Original Research Article
Pages 421-431
Nay-Wei Soong, Norman Arnheim
[37] Detection and quantification of point mutations in mitochondrial DNA by PCR Original Research Article
Pages 432-441
Makoto Yoneda, Yoshinori Tanno, Shoji Tsuji, Giuseppe Attardi
[38] Detection and quantification of oxidative adducts of mitochondrial DNA Original Research Article
Pages 442-453
Kenneth B. Beckman, Bruce N. Ames
[39] Use of fibroblast and lymphoblast cultures for detection of respiratory chain defects Original Research Article
Pages 454-464
Brian H. Robinson
[40] Use of myoblast cultures to study mitochondrial myopathies Original Research Article
Pages 465-475
Eric A. Shoubridge, Tim Johns, Louise Boulet
[41 ] Use of polarography to detect respiration defects in cell cultures Original Research Article
Pages 476-483
GГ¶tz Hofhaus, Rebecca M. Shakeley, Giuseppe Attardi
[42]Assessment of mitochondrial oxidative phosphorylation in patient muscle biopsies, lymphoblasts, and transmitochondrial cell lines Original Research Article
Pages 484-509
Ian A. Trounce, Yoon L. Kim, Albert S. Jun, Douglas C. Wallace
[43]Cytochemistry and immunocytochemistry of mitochondria in tissue sections Original Research Article
Pages 509-521
Monica Sciacco, Eduardo Bonilla
[44]Detection and analysis of mitochondrial DNA and RNA in muscle by in situ hybridization and single-fiber PCR Original Research Article
Pages 522-540
Carlos T. Moraes, Eric A. Schon
[45]Cytochemistry and immunocytochemistry of cytochrome-c oxidase at electron microscope level Original Research Article
Pages 540-555
J. Müller-Höcker, S. Schäfer
[46]Electrophoretic techniques for isolation and quantification of oxidative phosphorylation complexes from human tissues Original Research Article
Pages 555-566
Hermann Schagger
[47]Assays for characterizing URF 13, the pathotoxin and methomyl receptor of cms-T maize Original Research Article
Pages 566-581
David M. Rhoads, H.Carol Griffin, Barbara Brunner Neuenschwander, Charles S. Levings III, James N.Siedow
Author index
Pages 583-605
Subject index
Pages 607-621
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