Misbehaving Proteins: Protein (Mis)Folding, Aggregation, and Stability

✍ Scribed by Regina Murphy (Editor), Amos Tsai (Editor)

• Publisher: Springer
• Year: 2006
• Tongue: English
• Leaves: 348
• Edition: 1
• Category: Library

✦ Synopsis

This text provides an up-to-date collection of theoretical and experimental studies into protein folding, misfolding, aggregation, and stability. Additionally, issues faced during the development of protein products are illustrated. It contains an introductory chapter for readers new to the protein folding field. The book provides a thorough and clear discussion of computational approaches to understanding and modeling protein aggregation.

✦ Table of Contents

Cover......Page 1
Half-Title......Page 2
Inside Cover......Page 3
Copyright......Page 4
Preface......Page 5
Contents......Page 7
Part I - Introduction......Page 9
Protein Folding, Misfolding, Stability, and Aggregation......Page 10
Part II - Mathematical Models and Computational Methods......Page 21
Nonnative Protein Aggregation - Pathways, Kinetics, and Stability Prediction......Page 22
Simulations of Protein Aggregation......Page 52
Part III - Experimental Methods......Page 83
Elucidating Structure, Stability,and Conformational Distributions during Protein Aggregation with Hydrogen Exchange and Mass Spectrometry......Page 84
Application of Spectroscopic and Calorimetric Techniques in Protein Formulation Development......Page 102
Small-Angle Neutron Scattering as a Probe for Protein Aggregation at Many Length Scales......Page 127
Laser Light Scattering as an Indispensable Tool for Probing Protein Aggregation......Page 149
X-ray Diffraction for Characterizing Structure in Protein Aggregates......Page 168
Glass Dynamics and the Preservation of Proteins......Page 193
Part IV - Fundamental Studies in Model Systems......Page 215
Folding and Misfolding as a Function of Polypeptide Chain Elongation......Page 216
Determinants of Protein Folding and Aggregation in P22 Tailspike Protein......Page 245
Factors Affecting the Fibrillation of alpha-Synuclein, a Natively Unfolded Protein......Page 263
Molten Globule–Lipid Bilayer Interactions and Their Implications for Protein Transport and Aggregation......Page 284
Part V - Protein Product Development......Page 307
Self-Association of Therapeutic Proteins......Page 308
Mutational Approach to Improve Physical Stability of Protein Therapeutics Susceptible to Aggregation......Page 326
Index......Page 346