Minus strand RNA of hepatitis C virus liver tissues
β Scribed by Shibata, Motohiro; Takeda, Shin; Kudo, Toyoichiro; Kimura, Hiroshi; Kuzushima, Kiyotaka; Morishima, Tsuneo; Nagai, Yoshiyuki
- Book ID
- 123161480
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 131 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0168-8278
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## Abstract The presence of hepatitis C virus (HCV) RNA in serum and liver tissue was examined in seven patients with hepatocellular carcinoma (HCC), using the reverse transcriptaseβpolymerase chain reaction method with primers for the 5β²βnoncoding region. Plusβstrand HCVβRNA was detected in the se
Studies aimed at correlating the intrahepatic hepatitis C virus (HCV)-RNA level and anatomo-clinical features have been difficult because of sensitivity and specificity shortcomings of available techniques. We titered the genomic- and minus-strand HCV RNAs by a strand-specific, semiquantitative, gen
The combination of reverse transcription and polymerase chain reaction is a very powerful tool for the detection of hepatitis C virus RNA in sera of patients with hepatitis C virus infection. However, when studying the presence of this virus in tissue using polymerase chain reaction, it may be diffi