Minimization of plasma interferences by liquid chromatography with electrochemical detection after ultraviolet irradiation using chlordiazepoxide as a model substance

✍ Scribed by B. Ofner; R. Wintersteiger

• Book ID: 102985795
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 526 KB
• Volume: 305
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)00634-x

✦ Synopsis

Chlordiazepoxide was detected as a model substance in plasma by electrochemistry after separation by reversed-phase liquid chromatography. The method, which incorporates post-column, on-line UV irradiation, exploits the transformation of the molecule both into a phenolic hydroxyl and an oxaziridine by photolysis. For the determination in biological material, the optimization with respect to pH and working potential in combination with solid-phase extraction results in a decrease of plasma interferences. Selectivity and sensitivity can so be significantly improved. The detection limit was 3 ng/ml.