Minimal organic medium for suspension cultures of Paul's scarlet rose
โ Scribed by Kneeland K. Nesius; Laverne E. Uchytil; John S. Fletcher
- Book ID
- 104749155
- Publisher
- Springer-Verlag
- Year
- 1972
- Tongue
- English
- Weight
- 153 KB
- Volume
- 106
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
โฆ Synopsis
Maximum growth of suspension cultures of Paul's Scarlet rose has been demonstrated in a minimal organic medium possessing only 4 organic compounds: napthaleneacetic acid, kinetin, myo-inositol, and sucrose. Myo-inositol was not essential for growth, but sustained growth was reduced by 90% when it was omitted. Maximum growth required nitrate plus a supplemental amount of either NH 4 (+) or glutamine.
๐ SIMILAR VOLUMES
Growth and nitrate reductase activity were measured in Paul's Scarlet rose cell suspensions, cultured in media purified from molybdenum and containing nitrate or urea as sole nitrogen source with or without added Mo. Urea could replace nitrate to yield 80% of the fresh weight in nitrate medium. Nitr