The single cell gel electrophoresis, or comet assay, under alkaline conditions is a sensitive, simple and rapid method for the detection of DNA damage at the individual cell level. Its applicability as an indicator for the DNA crosslinking potency of a test substance was investigated in human white
Miniaturizing the comet assay with 3D vertical comets
β Scribed by Philippe Baert; Patrick Van Oostveldt
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 277 KB
- Volume
- 51A
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Ionising radiation induces DNA damage in the cells of the foodstu β . This fact was used to analyse DNA from single cells by agarose gel electrophoresis (DNA comet assay). The pattern formed by the DNA after electrophoresis (DNA comet) was visualised in a microscope, where hundreds of cells could be
The alkaline comet assay is a sensitive test for the detection of a variety of DNA lesions. However, crosslinks are not readily detected under standard test conditions. Recently, modifications have been introduced measuring crosslinks by determining the reduction of induced DNA migration. We used th