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Miniaturized asymmetrical flow field-flow fractionation: Application to biological vesicles

✍ Scribed by Sunok Oh; Dukjin Kang; Sung-Min Ahn; Richard J. Simpson; Bong-Hee Lee; Myeong Hee Moon

Book ID
102924596
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
640 KB
Volume
30
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Asymmetrical flow field‐flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size. By reducing the channel dimension, proteins or particulate materials can be separated within a few minutes without a significant loss in resolution. The mAFlFFF channel was applied for the separation of exosomes harvested from immortalized human mesenchymal stem cell line. It shows a potential to fractionate exosome vesicles according to sizes which can be useful for proteomic studies in relation to immunotherapeutic applications.

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