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Miniaturization of three carbohydrate analyses using a microsample plate reader

✍ Scribed by Jeffrey D. Fox; John F. Robyt


Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
357 KB
Volume
195
Category
Article
ISSN
0003-2697

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✦ Synopsis


Three carbohydrate analyses (reducing value by copper-bicinchoninate, total carbohydrate by phenol-sulfuric acid, and D-glucose by glucose oxidase) have been miniaturized using a microsample plate reader. The use of the reducing-value procedure to measure the hydrolysis of starch by alpha-amylase and the use of the glucose oxidase method to measure the hydrolysis of lactose by lactase are illustrated.