Microwave irradiation lowers immunohistological detection thresholds for p53 protein in squamous epithelium from non-neoplastic oesophagus
✍ Scribed by Darnton, S. Jane; Jenner, Keely
- Book ID
- 101222697
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 183 KB
- Volume
- 185
- Category
- Article
- ISSN
- 0022-3417
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✦ Synopsis
Mandard et al. report accumulation of p53, as shown by immunohistochemical staining, in uninvolved mucosa taken from 13 cases of resected, squamous carcinoma-bearing oesophagus. 1 Comment on their findings is also made in the Editorial of the same issue of the journal. 2 The antibody used was from clone DO7, after microwave antigen retrieval. There was expression of p53 in areas of chronic oesophagitis in uninvolved mucosa in all 13 cases. Since DO7 detects both accumulation of wild-type p53 and mutant p53, the authors rightly point out that accurate interpretation of the data needs molecular analysis of the p53 gene.
McKee et al. published a brief report in 1993 which showed clearly the effect of microwave antigen retrieval in lowering the immunohistological detection thresholds for p53 in basal and parabasal layers of the epidermis of UV-irradiated normal human skin. 3 Our Unit has recently studied the immunohistochemical expression of p53 protein in 22 samples of nonneoplastic oesophagus. Five of these were obtained at transplant harvest from patients with no gastrointestinal history, but who had been artificially ventilated. Seventeen samples were obtained at resection for benign disease. These comprised two cases of oesophageal spasm, each with a tight lower oesophageal sphincter and diverticulum; one case of achalasia; four cases of