Microwave-assisted extraction and in situ clean-up for the determination of fluoroquinolone antibiotics in chicken breast muscle by LC-MS/MS
✍ Scribed by Haoyan Xu; Ligang Chen; Lei Sun; Xin Sun; Xiaobo Du; Jian Wang; Tianyi Wang; Qinglei Zeng; Hui Wang; Yang Xu; Xiaopan Zhang; Lan Ding
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 280 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
A new method was developed for the determination of six fluoroquinolone antibiotics including fleroxacin, levofloxacin, ciprofloxacin, lomfloxacin, enrofloxacin, and sparfloxacin in chicken breast muscle, in which the extraction and clean‐up were performed in one step by microwave irradiation. The mixture of ACN containing 0.3% v/v phosphoric acid/water pH 3 (70:30, v/v) was used as the extraction solution and hexane was used as the clean‐up solution. The extract was analyzed by liquid chromatography–tandem mass spectrometry system. The RSDs of intra‐ and inter‐day obtained are in the range of 1.0–10.4 and 3.8–13.6%, respectively. In the three fortified levels of chicken breast muscle (20, 100, and 500 ng/g), the recoveries of fluoroquinolone antibiotics ranging from 66.0 to 97.2% are obtained. The LODs are in the range of 2.7–6.7 ng/g. This method simplifies the process of the sample preparation and reduces the operation errors.