Microvascular blood flow dynamics associated with photodynamic therapy, pulsed dye laser irradiation and combined regimens
✍ Scribed by Tia K. Smith; Bernard Choi; Julio C. Ramirez-San-Juan; J. Stuart Nelson; Kathryn Osann; Kristen M. Kelly
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 380 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0196-8092
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Background and Objectives
Previous in vitro studies demonstrated the potential utility of benzoporphyrin derivative monoacid ring A (BPD) photodynamic therapy (PDT) for vascular destruction. Moreover, the effects of PDT were enhanced when this intervention was followed immediately by pulsed dye laser (PDL) irradiation (PDT/PDL). We further evaluate vascular effects of PDT alone, PDL alone and PDT/PDL in an in vivo rodent dorsal skinfold model.
Study Design/Materials and Methods
A dorsal skinfold window chamber was installed surgically on female Sprague–Dawley rats. One milligram per kilogram of BPD solution was administered intravenously via a jugular venous catheter. Evaluated interventions were: control (no BPD, no light), PDT alone (576 nm, 16 minutes exposure time, 15 minutes post‐BPD injection, 10 mm spot), PDL alone at 7 J/cm^2^ (585 nm, 1.5 ms pulse duration, 7 mm spot), PDL alone at 10 J/cm^2^, PDT/PDL (PDL at 7 J/cm^2^), and PDT/PDL (PDL at 10 J/cm^2^). To assess changes in microvascular blood flow, laser speckle imaging was performed before, immediately after, and 18 hours post‐intervention.
Results
Epidermal irradiation was accomplished without blistering, scabbing or ulceration. A reduction in perfusion was achieved in all intervention groups. PDT/PDL at 7 J/cm^2^ resulted in the greatest reduction in vascular perfusion (56%).
Conclusions
BPD PDT can achieve safe and selective vascular flow reduction. PDT/PDL can enhance diminution of microvascular blood flow. Our results suggest that PDT and PDT/PDL should be evaluated as alternative therapeutic options for treatment of hypervascular skin lesions including port wine stain birthmarks. Lasers Surg. Med. © 2006 Wiley‐Liss, Inc.