✦ LIBER ✦

Microtubule organization in the apical cell ofSphacelaria(Phaeophyceae) and its related protoplast

✍ Scribed by A. M. Rusig; H. Guyader; G. Ducreux

Publisher: Springer
Year: 1993
Tongue: English
Weight: 449 KB
Volume: 260-261
Category: Article
ISSN: 1573-5141
DOI: 10.1007/bf00049016

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✦ Synopsis

The growth of the filamentous brown alga Sphacelaria depends on a large, strongly polarized, apical cell. The protoplast derived from this cell can be distinguished in a heterogeneous suspension by cytological markers, so it is possible to study development of the cytoskeleton during protoplast isolation and the first steps of regeneration. In the initial cell, microtubules show an asymmetric distribution along the axis; they are mainly located at the distal part around the physodes. After protoplast isolation, this polarity initially seems to be maintained; subsequently, the microtubules radiate from the two centrioles and spread out to the plasmalemma. This experimental model is suitable for investigating the development of the polarity of the initial cell, and the sequence of the first morphogenetic events leading to protoplast regeneration.

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