Microstructure of initial thrombus formation on foreign materials

✍ Scribed by Dutton, Robert C. ;Webber, A. J. ;Johnson, S. A. ;Baier, R. E.

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: English
• Weight: 714 KB
• Volume: 3
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.820030104

✦ Synopsis

By reflected light microscopy, the thrombi formed on a variety of foreign surfaces have been shown to be composed of isolated cellular aggregates and an interaggregate red cell fibrin mesh. The thrombi viewed by this method were seen in two dimensions as they formed at the foreign surface blood interface. Standard histological evaluation of the thrombi in the third dimension, that is in the depth dimension, revealed columnar shaped cellular aggregates extending from the foreign surface into the blood and a red cell fibrin mesh surrounding the aggregates. The thrombi formed a t Epon plate-blood interfaces were viewed with an electron microscope. Plates made of Epon embedding epoxy were used as the foreign surface exposed to blood, and the plates and fixed initial thrombi formed on them were embedded in more Epon. A layer of heavy metal staining, noncellular material was found covering the surface of the plates exposed to blood, with platelets deposited on this layer. Since platelets were not found adherent to the foreign surfaces directly, it is our postulate that the intervening film "conditions" the surface preliminary to platelet adhesion.