Microsomal oxidation of thiobenzamide. A photometric assay for the flavin-containing monooxygenase
โ Scribed by John R. Cashman; Robert P. Hanzlik
- Book ID
- 118316020
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 352 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0006-291X
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An assay system of flavin-containing monooxygenase was developed by fluorometric determination of benzydamine (BZY) N-oxidation with HPLC. The apparent Km value for the formation of BZY N-oxide from BZY by rat liver microsomes was similar to that by purified FMO. The Km and Vmax values for the forma
The K m value for tamoxifen is 1.2 mM for mouse FMO1 (human FMO1 is not expressed in adults) and 1.4 mM for human FMO3, with no detectable activity being expressed toward tamoxifen by FMO5 from either mouse or human. These data are derived from experiments using 3 H-tamoxifen as substrate in which t