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Microsomal alkaline phosphatase activity in retinol deficiency induced albino rats

✍ Scribed by Kaul, Sangeeta ;Krishnakantha, T. P.


Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
341 KB
Volume
37
Category
Article
ISSN
0027-769X

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✦ Synopsis


The investigations showed a significant decrease in the alkaline phosphatase activity of retinol deficient liver (48.6%), kidney (65.8%) and spleen (61.90/0), as compared to the controls (l0OYn). An increase in V,,, and K , by 12 to 51.5% and 90.4 to 189%, respectively, was observed in all the tissues in the retinol deficient group, as compared to the controls. Subsequent freezing and thawing reduced the activity of alkaline phosphatase by 22.5 to 35.8% in the experimental group; whereas the reduction in thc control group ranged from 8.8 to 21.5%. In the presence of lectins and detergents the activity of alkaline phosphatase decreased in both the groups to different levels.

Zusammenfassung

Aktivitit der mikrosomalen alkalischen Phosphatase in Albinoratten unter Retinolmangel Die Untersuchungen zeigten unter Retinolmangel, bezogen auf die Kontrollen (1 OOYn), eine signifikante Abnahme der Aktivitat der alkalischen Phosphatase der Leber (48,6%), der Nieren (65,8%) und der Milz (61,9%). In allen Geweben der Retinolmangelgruppe wurde, bezogen auf die Kontrollen, ein Anstieg von V,,, um 12 bis 51,5% und von K , um 90,4 bis 189% beobachtet. Aufeinanderfolgendes Gefrieren und Auftauen reduzierte in der experimentellen Gruppe die Aktivitat der alkalischen Phosphatase um 22,5 bis 35,8%, in der Kontrollgruppe um 8,8 bis 21,5%. In Gegenwart von Lcctincn und von Detergentien war die Aktivitit der alkalischen Phosphatase in unterschiedlichem MaR reduziert.


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