MicroRNAs in Development: Methods and Protocols (Methods in Molecular Biology, 732)

✍ Scribed by Tamas Dalmay (editor)

• Publisher: Humana
• Year: 2011
• Tongue: English
• Leaves: 232
• Category: Library

✦ Synopsis

Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, MicroRNAs in Development: Methods and Protocols serves as a practical guide for scientists of all backgrounds and conveys the appropriate sense of fascination associated with this vital field of research.

✦ Table of Contents

MicroRNAs in Development
Preface
Contents
Contributors
Chapter 1: In Situ Detection of microRNAs in Animals
Chapter 2: Detection of microRNAs in Plants by In Situ Hybridisation
Chapter 3: Detecting sRNAs by Northern Blotting
Chapter 4: Profiling MicroRNAs by Real-Time PCR
Chapter 5: Detection of Small RNAs and MicroRNAs Using Deep Sequencing Technology
Chapter 6: Detection of MicroRNAs in Prostate Cancer Cells by MicroRNA Array
Chapter 7: MicroRNA Knock Down by Cholesterol-Conjugated Antisense Oligos in Mouse Organ Culture
Chapter 8: Protocols for Use of Homologous Recombination Gene Targeting to Produce MicroRNA Mutants in Drosophila
Chapter 9: Engineering Elements for Gene Silencing: The Artificial MicroRNAs Technology
Chapter 10: Mimicry Technology: Suppressing Small RNA Activity in Plants
Chapter 11: Experimental Validation of MicroRNA Targets Using a Luciferase Reporter System
Chapter 12: Experimental Identification of MicroRNA Targets by Immunoprecipitation of Argonaute Protein Complexes
Chapter 13: Comprehensive Identification of miRNA Target Sites in Live Animals
Chapter 14: Target Validation of Plant microRNAs
Chapter 15: A High-Throughput Sequencing-Based Methodology to Identify All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes
Index