MicroRNA and Cancer: Methods and Protocols

✍ Scribed by Wei Wu (eds.)

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 222
• Series: Methods in Molecular Biology 1699
• Edition: 2
• Category: Library

✦ Synopsis

This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field

✦ Table of Contents

Front Matter ....Pages i-xi
Front Matter ....Pages 1-1
Noncoding RNAs in DNA Damage Response: Opportunities for Cancer Therapeutics (Wani Arjumand, Asia Asiaf, Shiekh Tanveer Ahmad)....Pages 3-21
MicroRNAs in Breast Cancer: Diagnostic and Therapeutic Potential (Asia Asiaf, Shiekh Tanveer Ahmad, Wani Arjumand, Mohammad Afzal Zargar)....Pages 23-43
Involvement of miRNAs and Pseudogenes in Cancer (Lütfi Tutar, Aykut Özgür, Yusuf Tutar)....Pages 45-66
MicroRNAs Reprogram Tumor Immune Response (Wei Cao, Wenfang Cheng, Wei Wu)....Pages 67-74
Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like Gene Expression, RNA Editing, and MicroRNAs Regulation (Wei Cao, Wei Wu)....Pages 75-81
MicroRNAs Change the Landscape of Cancer Resistance (Jun Zhu, Wei Zhu, Wei Wu)....Pages 83-89
MicroRNA, Noise, and Gene Expression Regulation (Wei Wu)....Pages 91-96
Front Matter ....Pages 97-97
Deep Sequencing Reveals a MicroRNA Expression Signature in Triple-Negative Breast Cancer (Yao-Yin Chang, Liang-Chuan Lai, Mong-Hsun Tsai, Eric Y. Chuang)....Pages 99-111
Detection of Plasma MicroRNA Signature in Osteosarcoma Patients (Wendy Allen-Rhoades, Jason T. Yustein)....Pages 113-118
Identification of E6/E7-Dependent MicroRNAs in HPV-Positive Cancer Cells (Anja Honegger, Daniela Schilling, Holger Sültmann, Karin Hoppe-Seyler, Felix Hoppe-Seyler)....Pages 119-134
Combination of Anti-miRNAs Oligonucleotides with Low Amounts of Chemotherapeutic Agents for Pancreatic Cancer Therapy (Marta Passadouro, Henrique Faneca)....Pages 135-154
Evaluation of MicroRNA Delivery In Vivo (Rikki A. M. Brown, Kirsty L. Richardson, Felicity C. Kalinowski, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir et al.)....Pages 155-178
Angiogenesis Analysis by In Vitro Coculture Assays in Transwell Chambers in Ovarian Cancer (Ali Flores-Pérez, Dolores Gallardo Rincón, Erika Ruiz-García, Raquel Echavarria, Laurence A. Marchat, Elizbeth Álvarez-Sánchez et al.)....Pages 179-186
Application of Individual qPCR Performance Parameters for Quality Control of Circulating MicroRNA Data (Anna Brunet-Vega, María Elisa Quílez, María José Ramírez-Lázaro, Sergio Lario)....Pages 187-199
Construction of Multi-Potent MicroRNA Sponge and Its Functional Evaluation (Suhwan Chang)....Pages 201-209
MicroRNA Sequencing Data Analysis Toolkits (Wei Wu)....Pages 211-215
Back Matter ....Pages 217-222

✦ Subjects

Cancer Research