MicroRNA-206 Is Associated With Invasion and Metastasis of Lung Cancer

✍ Scribed by Xiaochen Wang; Chunhua Ling; Yanyan Bai; Jun Zhao

• Publisher: Wiley (John Wiley & Sons)
• Year: 2010
• Tongue: English
• Weight: 476 KB
• Volume: 294
• Category: Article
• ISSN: 1932-8486
• DOI: 10.1002/ar.21287

✦ Synopsis

Abstract

MicroRNAs are novel small noncoding RNA molecules that regulate gene expression at the post‐transcriptional level. Compelling evidence reveals that there is a causative link between microRNAs deregulation and cancer development and progression. The present study aims to explore the function of miR‐206 in the proliferation, apoptosis, motility, and invasion of nonsmall cell lung cancer. Using real‐time PCR, we detected the miR‐206 expression of normal lung tissues, tumor tissues, human normal bronchial epithelial cell line, and six lung cancer cell lines (LCCLs). Then, we evaluated the role of miR‐206 in cell proliferation, apoptosis, and invasion using Cell Counting Kit‐8 assay, Annexin‐V/FITC assay, wound healing, and Transwell assay in LCCLs. As a result, miR‐206 expression level was lower in high metastasis tumors and 95D than low metastasis tumors and normal lung tissues as well as other LCCLs. After miR‐206 was upregulated in LCCLs, cell proliferation was notably attenuated and apoptosis was significantly increased. Furthermore, overexpression of miR‐206 inhibited migration and invasion of lung cancer cells. In conclusion, our data suggest that expression level of miR‐206 was inversely correlated with metastatic potential of lung cancer. Anat Rec, 2010. © 2010 Wiley‐Liss, Inc.