MicroRNA-125b suppressesed human liver cancer cell proliferation and metastasis by directly targeting oncogene LIN28B
✍ Scribed by Linhui Liang; Chun-Ming Wong; Qiao Ying; Dorothy Ngo-Yin Fan; Shenglin Huang; Jie Ding; Jian Yao; Mingxia Yan; Jinjun Li; Ming Yao; Irene Oi-Lin Ng; Xianghuo He
- Book ID
- 102850077
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 538 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0270-9139
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✦ Synopsis
MicroRNAs (miRNAs) are small, noncoding RNAs that can act as oncogenes or tumor suppressors in human cancer. Our previous study showed that miR-125b was a prognostic indicator for patients with hepatocellular carcinoma (HCC), but its functions and exact mechanisms in hepatic carcinogenesis are still unknown. Here we demonstrate that miR-125b suppressed HCC cell growth in vitro and in vivo. Moreover, miR-125b increased p21Cip1/Waf1 expression and arrested cell cycle at G₁ to S transition. In addition, miR-125b inhibited HCC cell migration and invasion. Further studies revealed that LIN28B was a downstream target of miR-125b in HCC cells as miR-125b bound directly to the 3' untranslated region of LIN28B, thus reducing both the messenger RNA and protein levels of LIN28B. Silencing of LIN28B recapitulated the effects of miR-125b overexpression, whereas enforced expression of LIN28B reversed the suppressive effects of miR-125b.
Conclusion:
These findings indicate that mir-125b exerts tumor-suppressive effects in hepatic carcinogenesis through the suppression of oncogene lin28b expression and suggest a therapeutic application of mir-125b in hcc.