โ Scribed by Howard B. Gutstein; Jeffrey S. Morris; Suresh P. Annangudi; Jonathan V. Sweedler
No coin nor oath required. For personal study only.
Proteomics, the largeโscale study of protein expression in organisms, offers the potential to evaluate global changes in protein expression and their postโtranslational modifications that take place in response to normal or pathological stimuli. One challenge has been the requirement for substantial amounts of tissue in order to perform comprehensive proteomic characterization. In heterogeneous tissues, such as brain, this has limited the application of proteomic methodologies. Efforts to adapt standard methods of tissue sampling, protein extraction, arraying, and identification are reviewed, with an emphasis on those appropriate to smaller samples ranging in size from several microliters down to single cells. The effects of miniaturization on these analyses are highlighted using neuroscienceโrelated examples, as are statistical issues unique to the highโdimensional datasets generated by proteomic experiments. ยฉ 2008 Wiley Periodicals, Inc., Mass Spec Rev 27: 316โ330, 2008
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