Micropropagation ofMorus laevigataWall. from mature trees
โ Scribed by M. Hossain; S. M. Rahman; A. Zaman; O. I. Joarder; R. Islam
- Book ID
- 104676500
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 423 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0721-7714
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โฆ Synopsis
Multiple shoots were obtained from nodal explants of 10-year-old tree of Morus laevigata on Murashige and Skoog's medium supplemented with different concentrations (0.5-5.0 mg.l(-1)) of benzyladenine (BA). Nodal segments taken from in vitro proliferated shoots gave further multiple shoots when cultured on the same basal medium containing 2.5 mg.l(-1) BA. Repeated subculture resulted in rapid shoot multiplication at the average rate of 6-fold per subculture. In vitro raised shoots rooted on MS medium containing 0.1 mg. l(-1) each of 3-indolebutyric acid (ISA) and ฮฑ-naphthaleneacetic acid (NAA). The regenerated plantlets were successfully established in soil under field conditions after a few days of indoor acclimatization.
๐ SIMILAR VOLUMES
Multiple shoots were induced from nodal segments of mature trees of Ulmus pumila L. on Murashige and Skoog (MS) medium supplemented with benzyladenine (BA). Further multiple shoots were obtained from nodal segments taken from in vitro proliferated shoots when cultured in MS medium containing 0.5 mg.
In vitro propagation protocols were established for two leguminous trees, Bauhinia variegata and Parkinsonia aculeata. In each case axillary shoot proliferation was achieved from nodal explants from mature (6-8 years) trees using Murashige & Skoog's medium supplemented with 2.22-31.1 IxM of 6-benzyl